Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Low-affinity CCK-A receptors are coexpressed with leptin receptors in rat nodose ganglia: implications for leptin as a regulator of short-term satiety
doi: 10.1152/ajpgi.00356.2010
Figure Lengend Snippet: Effects of JMV-180 on CCK- and leptin-induced c-Fos expression in rat nodose ganglion. Triple fluorescence labeling of rat nodose ganglia shows c-Fos-positive neuronal nuclei (red), CCKAR (blue), and OB-Rb (green). A: c-Fos expression in nodose ganglion in response to intraperitoneal (IP) injection of CCK-8 (3.5 μg/kg) and leptin (120 μg/kg). B: c-Fos expression in nodose ganglion treated with JMV-180 (350 μg/kg ip) 15 min before IP injection of CCK-8 (3.5 μg/kg) and leptin (120 μg/kg). JMV-180 reduced c-Fos-IR in the nodose ganglion. Scale bar: 100 μm. C: histogram shows percentage of neurons that exhibited c-Fos immunoreactivities over the total number of neurons in the nodose ganglion (n = 3). Note that JMV-180 significantly reduced c-Fos expression in the neurons expressing CCKAR and those expressing both CCKAR and OB-Rb. *P < 0.01 compared with no pretreatment with JMV-180.
Article Snippet: Sections were washed in PBS buffer three times and incubated with c-Fos rabbit antibody (Ab-5) obtained from Calbiochem diluted in 1:2,000 and one or both of the following antibodies: goat anti-CCKAR polyclonal antibody (1:200, SC-16172, Santa Cruz, CA) and/or mouse anti-OB-Rb (B-3) monoclonal antibody (1:250, SC-8391, Santa Cruz).
Techniques: Expressing, Fluorescence, Labeling, Injection, CCK-8 Assay